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Additional Biology Resume Samples
Molecular Biologist Resume Samples
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0-5 years of experience
Conducted study to reclassify the rat model to determine if the organism is a proper model for human breast cancer prognosis
- Collaborated with three other team members for the duration of the extensive study
- Analyzed data from Ph.D research associate
- Assisted research associate with bench work, collection, and analysis of data
- Developed a passion for oncology and genuine interest in breast cancer on a personal level
0-5 years of experience
Under a federal contract, performed environmental testing in order to rapidly identify bacterial and viral public health threats.
- Performed and interpreted multiple real-time PCR assays in order to detect and characterize potential pathogens and ensure assay quality.
- Maintained chain of custody and sample integrity from DNA extraction through qPCR using aseptic BSL2 techniques.
- Followed regulatory requirements to develop daily reports and document findings into laboratory information systems.
0-5 years of experience
Focused on effect of CRP and complement system in gut ischemia/reperfusion injury.
- Discovered CRP augments intestinal ischemia damage with increased complement 3 activation, decay accelerating factor (CD55) attenuates gut insults via suppression of complement activity.
- Conducted hemorrhage shock and apoptosis, NF-kb signal transduction.
- Analyzed brain trauma and apoptosis pathway.
0-5 years of experience
Routinely performed conventional, Real-Time, and Reverse Transcriptase (RT) PCR with regard to enteroviruses
- Performed water filtration and secondary virus concentration methods
- RNA and DNA extractions for samples as well as inhibitor removal procedures
- Maintained a clean and efficient laboratory setting, including calibration of instruments such as thermocyclers
- Maintained scientific supply inventories via computer-based spreadsheets
- Conducted supplemental scientific literature research and presented data
0-5 years of experience
Successfully planned a quality control program as well as a genotypic profiling start up lab and created a workflow for sample processing
- Lead research and development team to design protocols for several projects aimed at isolating fetal cells from maternal peripheral circulation using flow cytometry.
- Evaluated and optimized sperm DNA extraction techniques.
- Provided technical expertise and served as task lead in support of internal programs
6-10 years of experience
Conducted bacterial and rice transformation experiments with the C4 genes from different C4 species.
- Planned and executed molecular characterization of the regenerated transgenic plants for transgene integration, expression and copy number.
- Examined cell specificity of different gene promoters using GUS reporter assays.
- Performed data generation, collection, documentation, analysis and presentation.
- Managed laboratory functions including organization, ordering of supplies and scheduling equipment use.
- Conducted group and intergroup monthly meetings to report research progress.
- Conducted web meetings to maintain good communication flow and data sharing with the C4 rice collaborators at Cambridge, Oxford, Toronto, Germany and CSIRO.
- Supervised and trained 15 research staff and 6 graduate students.
- Prepared bimonthly progress update reports, highlight reports and annual reports.
- Attended and presented research results in international conferences.
- Wrote research and review articles for publication in peer reviewed journals.
0-5 years of experience
Experimental design cloning and transformation of DNA cassettes (plasmid and integrant) into S. cerevisiae and E. coli strains.
- Daily PCR (SOE, multiplex, touchdown and quantitative), restriction digest, electrophoresis, and spectrophotometric analysis.
- Protein assays and GC analysis of cellular metabolites for strain evaluation.
- Optimized electroporation protocol for S. cerevisiae, increasing transformation efficiency by five fold in several strains.
- Oversight of company-wide strain and culture collection and maintenance of AATI Fragment Analyzer and Qiagen QIAcube.
- Responsible for high-throughput strain development, screening, fermentations, and protein assays utilizing various genes to enhance isobutanol production in S. cerevisiae.
- Responsible for writing SOPs, technical strain development, and plasmid construction reports in addition to maintaining detailed laboratory notebook.
6-10 years of experience
Perform HIV-1 RNA 3.0 Assay by Branched DNA (bDNA) by using Bayer System 340 bDNA Analyzer.
- Perform HIV Genotyping by using Bayer Trugene System.
- Perform Rapid Plasma Reagin (RPR), CBC using Beckman Coulter Act dif. 2, and flow cytometry CD4 (T-CELL) using Becton Dickinson Facs count.
- Perform chemistry panels using ACE by Alfwasserman analyzer and PSA and TSH using IMX Immunoassay analyzer.
- Monitor quality control and perform proficiency laboratory testing.
0-5 years of experience
Developed strategies for pathway engineering of yeast strains for metabolite production
- Designed and performed standard molecular biology assays
- Performed bioinformatic analysis of nucleic acid and protein sequence data
- Wrote technical reports of completed projects
0-5 years of experience
Performed molecular biologist tests following the scientific procedures for the Apiaceas plant family. Promoted and performed the use of the PCR platform and Electron Microscope for the analysis of different species. Performed analysis for the amplification and quantification of samples using the PCR system. Performed the digitalization of several samples for the groups Ericaceae and Xyriaceae.
Key Contributions:
- Contributed to the amplification and quantification of several samples using the PCR system.
- Maintained and digitalized in a daily basis of several collection samples.
- Contributed to the re-organization and integration of an electronic herbarium (NYBG electronic herbarium).
0-5 years of experience
Isolate endothelial and vascular smooth muscle cells from rat mesenteric arteries using Laser Capture Microdissection (LCM).
- Quantitate and determine RNA integrity of LCM captured cells using Ribogreen and Agilent Bioanalyzer2100, for subsequent RNA amplification and Affy Metrix analysis.
- Effectively communicate/coordinate activities with departmental personnel to maintain LCM study activities.
- Identify problems, troubleshoot and take corrective actions to rectify instrumentation/sample issues.
- Record and maintain study data according to departmental SOP and protocol guidelines.
0-5 years of experience
- Detected and characterized respiratory viruses such as Influenza and Adenoviruses.
- Completed the validation and implementation of the Luminex multiplex x-TAG RVP assay and the Qiagen MDX extraction instrument.
- Sequenced, analyzed, interpreted and reported over 3000 global influenza isolates during two influenza seasons.
- Implemented reverse transcription polymerase chain reaction (RT-PCR) clinical diagnostics assay to detect Influenza A/H1, Influenza A/H3, Influenza B and Adenovirus 14 in original patient specimens.
- Maintained and adhered to a quality control program with guidelines established by the College of American Pathologists (CAP).
- Trained new personnel within the section.
- Member of the team that isolated and characterized the 2007 influenza A/H1N1 strain that was selected as the 2009 Southern Hemisphere vaccine by the CDC-WHO.
- Team member that collaborated with National Institutes of Health (Genbank) to build a DOD influenza sequence data pipeline.
0-5 years of experience
- Developed a quantitative LCx probe assay for HIV detection used in blood screening and drug therapy monitoring.
- Developed LCx Probe assay for detection of newly discovered Hepatitis GBV-C virus. The assay was launched worldwide as the first available GBV-C research use kit in the market.
- Established a new standard procedure and documentation system for manufacturing Research Use Only (RUO) Kit via coordination with R&D and Operations.
10+ years of experience
Innovator in global healthcare providing high quality testing and regulated biological products
- Perform molecular biology methods for RNA and DNA replication using Roche and Tigris GenProbe reagents for detection of HIV, HCV, HBV, and West Nile Virus
- Participate in NAT team to focus on streamlining laboratory workflow
- Ensure compliance cGMP, ISO, and FDA regulations for all inspections
- Train junior level staff on usage of Tigris and Tecan
- Review and update standard operating procedures for quality assurance
0-5 years of experience
Performed high throughput DNA extraction processes using the following methods: phenol/chloroform method, Power Food MoBio DNA Kit, Qiagen DNeasy Plant Mini kit, and the Qiagen DNeasy Blood and Tissue kit
- Performed Plant Tissue Test using conventional PCR and the Tissue Lyser on cucurbit samples
- Responsible for analyzing high volumes of data for presence of pathogenic DNA, and qPCR inhibition.
- Involved in a primer validation project that screened 22 novel primers against >500 pathogenic DNA isolates for optimal primer specificity and sensitivity
- Proficient in use with cutting edge qPCR units and respective software’s from Roche and Life Technologies
- Trained new employees on the Standard Seedwash Assay, DNA extraction, and qPCR process.
- Responsible for inventory management and sample organization.
- Trained on using the Hamilton STAR automated liquid handler
- Followed SOP’s in conformance with international production standards.
0-5 years of experience
Performs manual extraction and purification of DNA from Oragene DNA saliva collection tubes and Quantification of DNA via spectrophotometer.
- Performs automated extraction, quantification, normalization of saliva samples using Tecan Freedom Evo extractor system.
- Execute whole genome genotyping using the automated Illumina Infinium HD (SNP) assay and Illumina GoldenGate Assay, Amplification, Resuspension, Hybridization, primer Staining of DNA,
- Thorough knowledge of Illumina BeadChip & iScan platform for high density SNP microarray screening.
- Extensive experience with robotic manipulation of patient DNA using Tecan platform
- Team Lead. Provide technical guidance to laboratory assistants and assists in training of new employees.
0-5 years of experience
Real-Time PCR testing to identify and characterize potential pathogenic bacterial and viral threat agents
- Process and triage of biological samples while maintaining chain of custody
- Perform and interpret a variety of specialized tests and strains, detection, and identification of pathogenic microorganisms
- Prepares written and oral reports, answers questions, troubleshoots and makes recommendations to the supervisor for inclusion in comprehensive reports on test findings
- Communicates changes in equipment to Logistics Manager
- Writes and reviews standard operating procedures within guidelines of laboratory
- Participates in cross training related to bioterrorism and other public health emergencies (BSL-3 Training)
- First responder (24/7) in emergency situations
- Fosters positive relations with Public Health Laboratory by participating in committees and events
0-5 years of experience
Project: Bridging and optimization of the Laboratory Response Network (LRN) real-time PCR assays from ABI7500 DX to the Cepheid SmartCycler
- Optimize and validate real-time PCR assays for the rapid detection and identification of viruses.
- Analyze, prepare, and present test results to team members.
- Investigate and report unexpected test results.
- Attend CDC division, branch, and project planning meetings.
- Provide technical consultation and contribute to overall project direction.
- Maintain CDC Select Agent training and compliance.
0-5 years of experience
Developed a proprietary vector for construction of antibody phage display library.
- Developed a comprehensive set of oligonucleotide primers for antibody sequence assembly.
- Developed a novel bacteria strain to increase the antibody repertoire in a library.
- Developed a novel cloning strategy to increase the antibody repertoire in an antibody phage library.
0-5 years of experience
- Ran daily PCRs for potential pathogenic bacterial and viral agents
- Performed DNA extractions using a vacuum filtration method
- Maintained a chain of custody for sample receiving and processing
- Knowledgeable in the proper handling, storage and disposal of biological agents for a BSL 2
- Maintained QA/QC procedures and guidelines for the laboratory
0-5 years of experience
[company name]. used recombinant DNA technology and protein engineering to develop modified
plant varieties for use in animal nutrition, biofuels, chemical production, and commercialization of industrial enzymes.
- Constructed plasmid libraries with modified genes using overlap-extension PCR and site- directed mutagenesis
- Performed molecular cloning by homologous recombination and restriction/ligation
- Cultured and transformed S. cerevisiae, P. pastoris, and E. coli
- Expressed recombinant genes in microbial systems
- Extracted and isolated proteins from yeast, bacteria, and corn embryos
- Examined proteins by western blot and enzymatic activity assay
- Designed PCR primers for amplification, cloning, and mutagenesis
- Catalogued and analyzed DNA sequences using Vector NTI (Life Technologies, Inc.) and BLAST (NCBI)
- Presented data at internal progress update meetings
- Technical methods: bacterial and yeast culture; PCR (conventional and overlap- extension), primer design, molecular cloning by restriction/ligation and homologous
6-10 years of experience
Provide technical support for research projects in the Microbial Exposure Research Branch and Molecular Ecology Research Branch of the US EPA. Design and execute daily experiments, develop SOPs, and perform data analysis in support of research activities. Research focused on the identification and characterization of bacterial pathogens found in drinking water systems and recreational water sources.
- DNA and RNA extractions for genomic analyses
- Design, optimization, and validation of qPCR assays for pathogen detection and quantification
- RT-qPCR and microarray analysis of virulence effector genes associated with water pathogens
- 16S and 18S rRNA gene amplification, cloning and sequencing
- Preparation and sequencing of DNA samples on ABI 3730XL
- Responsible for the development, validation and publishing of SOPs
- Developed Quality Assurance Activity Plan and wrote Health and Safety protocols and Work Plans for Work Assignments
- Assist with the statistical analyses of results and the writing and publication of research.
0-5 years of experience
National Molecular Laboratory, [company name]
- Genotyped blood group using PCR
- Constructed vectors for human RhD and Rhce transgenic mice and genotyped offspring
- Investigated regulation mechanism of the ammonium transporting function of Rh family
- Explored the physiological function of Kx with Xenopus oocyte expression system and two electrode voltage clamp system
0-5 years of experience
- Extracted DNA and ran PCR from samples of amniotic fluid, blood, and tissue.
- Performed DNA Sequencing and Fragment Analysis by Capillary Electrophoresis.
- Performed Gel Electrophoresis.
- Assisted with the introduction, array development, and analysis for Array Comparative Genome Hybridization for the laboratory.
0-5 years of experience
Set up a system to express mammalian proteins in Sf9 cells
- Constructed adenoviral expression constructs of Perilipin A
- Provided assistance in training graduate and undergraduate students
- Purchased lab supply and equipment
- Maintained lab inventory
0-5 years of experience
Research and Development Department
- Characterized microbes, enzymes, and raw ingredients to enhance dry specialty livestock products
- Supported sales staff and clients in the field
- Conducted field trials, as part of sales support, with dry specialty products, on poultry, swine, and dairy farms
0-5 years of experience
Cloning into E. coli and industrial yeast strains, pathway engineering
- Yeast cell culture, sterile technique and high throughput culture (Liquid handling using Tecan robot to set up HT-growth assays in 96 well plates).
- Walk up GC analysis, LC analysis, FPLC, protein purification
- Quantitative western blotting, enzyme assays, SOE PCR, integrations
- Yeast mating, sporulation and tetrad dissection as well as random spore analysis
0-5 years of experience
Molecular Diagnostics
- Responsible for extraction, cell count, freezing and storage of Peripheral Blood Mononuclear Cells (PBMCS)
- Performs cell counts using Siemans Advia instrumentation.
- Performs manual DNA extraction using Qiagen reagents.
- Assist with the training of new technologists.
- Assist with the validation of new equipment.
0-5 years of experience
- Worked in an environment where procedures and standards are compliant with US FDA regulations
- In-depth knowledge of ABI 3730 DNA Analyzer for DNA sequencing
- Performing Maxi-preps, Mini-preps and Direct-colony sequencing reactions.
- Maintaining lab inventories, mediums and chemical stocks for the smooth functionality of the lab.
- Troubleshooting problematic sequencing reactions and equipment related problem
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[Molecular Biologist]
Last edited by: Gabriela Barcenas, CPRW, Content Writer II -
Gabriela Barcenas
CPRW, Content Writer IIGabriela is a Certified Professional Resume Writer (CPRW) and career adviser. With 10 years of digital media experience and five years of resume writing expertise, her publication history includes fashion, education, travel, social justice, equitable design and career advice.
